We are interested to use the Aber Futura Biomass probe (Dielectric spectroscopy) to measure the capacitance data of E.Coli and also scanning data of E.coli cell parameters (Cole-Cole alpha,Cell Diameter,Biovolume, Conductivity etc...). We are using bacterial mode(Default: 1.1MHz) with Polarisation OFF but we couldn't able to measure the capacitance data using default mode. Our offline data suggested that cells are growing well. Anyone can help us to solve this issue.
Set the probe to zero first using the cell free media. then using the known amount of cells in the media calibrate the probe. for proper calibration use different amount of known cells in in media in different tubes or flask and make a standrad curve with no of cells against capistance measured. use the proper mode and then in the last dont forgot to click on start logging, with this it will start recording the data in a excel sheet.
aber generates a huge amount of data.
Thank you very much for your response. We have done that....E.coli cells are small in size so capacitance values are not sensitive when cells are low. Are you able to measure the capacitance for biomass 0-20g/L from the inoculation in the bioreactor?
Hi,
Aber Futura, work with two types of on-line biomass measurements, optical and capacitance. The second one, takes a measurement of available cells in the medium. You should ask them, the range of capacitance depending of your type of cells and the type of medium (sometimes there a lot of nutrients that can affect the capacitance measurements).
In the attached file, you can have an idea of this.
Best Regards.
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