Hi,
I would like to measure the fitness of bacterial species carrying antibiotic resistance genes (two genes in total) and model the evolution and spread of these two strains (with two different antibiotic resistant genes). Is there any protocol to measure fitness for modelling evolution experiments? I have flow cytometer in our lab. Please share the links for protocols or similar experiments.
Thank you.
At the risk of sounding repetitive, here is a recent article whose reading could be inspiring: Baym et al. 2016. Spatiotemporal microbial evolution on antibiotic landscapes. Science 353: 1147-51.
I also found very useful the following review: Ghoul & Mitri. 2016. The ecology and evolution of microbial competition. Trends in Microbiology 24: 833-45.
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