How to manipulate the transition from exponential to stationary phase in mammalian cell culture?

03 April 2020 0 10K Report

Is there a way to stop exponential growth and increase protein production, without using chemical inducers?

What can happen if one increases/decreases the concentration of nutrients in the medium at a stage of the process?

More Giacomo Pesci's questions See All

How frequently do polymerases make mistakes?

I used the Gibson assembly to join together two PCR fragments (4400 bp +770 bp). After transformation of E. coli with the Gibson mix, I isolated DNA from single colonies and I performed a...

28 March 2020 1,443 3 View

Which are the most effective ways to promote oxidative metabolism during cell culture?

I am looking for chemicals that can be used in mammalian cell culture to enhance oxidative metabolism (TCA cycle). Can NAD+ precursors (NR, for example) be used?

17 March 2020 7,494 4 View

Purification of fusion protein with Protein L affinity column?

I am studying a single-chain variable fragment (scFv) linked to a luciferase (Nanoluc) at the C-terminus (N-scFv-linker-Nluc-C). The naked scFv (not linked to Nluc) can be easily purified using...

22 February 2020 9,149 2 View

Purification of fusion protein with Protein L affinity column?

I am studying a single-chain variable fragment (scFv) linked to a luciferase (Nanoluc) at the C-terminus (N-scFv-linker-Nluc-C). The naked scFv (not linked to Nluc) can be easily purified using...

01 February 2020 6,251 2 View

Why do my minipreps look like this?

I did several minipreps (different plasmids extracted from different cell lines) and when I run them on agarose gel 0.8% they don't look good (smear at high MW and it looks always like that). The...

05 June 2019 9,887 5 View

How to prepare bacterial conditioned media to study the effect of bacterial secretome?

I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...

17 July 2024 3,785 9 View

How is the preparation to obtain secondary metabolic extracts?

Hi everyone! I am working with bacteria and fungal collected from marine sediments. I would to know the methodology since the preparation of biomass until the extraction and stored of secondary...

03 July 2024 6,177 3 View

Could cell metabolism change the cell morphology?

Hi, I have been overexpressing a gene in the cells, and I have found that the genes could promote cell metabolism. Curiously, the cell morphology enlarges. How can I explain this phenomenon? What...

09 May 2024 4,308 2 View

How does CKD affect drug metabolism and elimination, and what considerations should be taken into account when administering anesthesia to CKD patien?

How does CKD affect drug metabolism and elimination, and what considerations should be taken into account when administering anesthesia to CKD patients?Optimizing Perioperative Care for Patients...

17 April 2024 8,059 1 View

Describe the pharmacokinetic and pharmacodynamic changes that affect drug metabolism and response in pregnant patients?

Describe the pharmacokinetic and pharmacodynamic changes that affect drug metabolism and response in pregnant patients. Pharmacokinetic and pharmacodynamic changes occur during pregnancy,...

09 April 2024 8,433 1 View

Why IBMX but not forskolin during adipocyte differentiiation?

During preadiopcyte in vitro differentiation, cocktail stimuli is needed. But why IBMX but not forskolin? Both of them can increase the intracellular level of cAMP and lead to PKA pathway...

28 February 2024 8,146 0 View

Are there specific biochemical tests designed to analyze the ADMET properties of a particular compound?

Are there specific biochemical tests designed to analyze the ADMET properties of compounds, providing insights into their Absorption, Distribution, Metabolism, Excretion, and Toxicity?

25 December 2023 4,699 2 View

Finding and doing phylogenetic statistics on discrete variables?

Hello everyone, I hope everyone is doing well and is keeping safe. I was wondering if someone could please help me in that I want to use statistical analysis to measure whether different genera...

30 October 2023 7,035 0 View

Bacterial metabolic profile?

Hi. I have a bacteria that has antagonistic properties. I would like to find out what biochemical compounds are responsible. Are there companies that do this? Maybe someone knows the pricing?

27 October 2023 8,513 7 View

CALL FOR PAPERS—Special issue on "Advances in Transcriptomics in Tumor Metabolism"?

Submission Deadline: 30 September 2024 For more detail: https://www.techscience.com/or/special_detail/tumor-metabolism Summary Metabolism refers to the unique biochemical processes that sustain...

17 October 2023 5,018 1 View