How to make the best PRP for biostimulation in hair loss?

More Barry Dekeyser's questions See All

Is there any running studies that have looked at the correlation between step rate and step height?

Is there any research on the effect of step rate manipulation on step height?

13 August 2020 2,641 1 View

How to report a post-hoc comparison for robust mixed ANOVA?

Hi all I have conducted a robust mixed design ANOVA using WRS2 in R. My data are time taken in seconds, and not normally distributed. I cannot remove outliers as the experiment is based around...

23 July 2020 6,766 4 View

Understanding the theory of quarter wavelength coatings?

I remember asking this as an undergraduate physics student and got the answer "it's a property of light", so maybe things have changed in 40 years... So coatings cause reflections at the surface...

30 May 2020 7,691 9 View

Creating randomization matrices (null models) with abundance data in R (Ulrich & Gotelli 2010)?

Hello, We are aiming to create a simple randomization algorithm in R program with abundance data. Specifically, we are aiming to create the IT-Algorithm (Ulrich & Gotelli 2010, Ecology),...

25 March 2020 5,873 3 View

What are some examples of practice based methods and techniques that can be used to study social dance styles?

I am currently developing a project where the primary mode of inquiry is rooted in practice based methods and techniques. The project is focused on a social dance form. I have read several...

23 March 2020 793 5 View

How does being a teacher influence creative processes in creating choreography?

I would be interested in resources that pertain to how identity or the different roles we possess in dance making (dancer, teacher, spectator, mentor, etc.) influence creative processes in...

06 December 2019 8,064 2 View

OpenSees DisplacementControl of a node that is not loaded?

I am trying to model nonlinear, cyclic behaviour of a cantilever beam, I wish to apply a cyclic load at the free end. And I want the midpoint of the beam to be displacement controlled (ie. reverse...

17 October 2019 4,895 1 View

Ecological Validity: How can experiments that take place in a social setting measure validity and reliability?

Thinking in terms of a social setting such as a dance, a concert, a meal, if an experiment were to be designed in such a way, how can the method be validated? Similarly, what role would...

26 August 2019 3,415 3 View

Does the volume of a song influence our enjoyment of it?

If a song is played louder does it increase or decrease our enjoyment towards a song? Do we have to already enjoy or dislike a song in order to notice the influence of volume? Any possible...

22 August 2019 2,089 11 View

Does the release of certain chemicals diminish as we continue to listen to our once favorite song?

For the past few months I have been thinking through how our favorite songs become our favorite songs-- an experience, instrumentation, lyrics, etc. I am curious as to what happens on the chemical...

13 August 2019 5,434 2 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Chris Blatchley

I've written several answers to this on similar questions.... :)

Barry Dekeyser

After testing 2 commercial kits we measure that in some patients after centrifugation of the whole blood, we are not able to create real PRP. WE ARE NOT ABLE TO CONCENTRATE THE PLATELETS, despite goof quality kits and centrifugation.

All suggestions to help us working on this are welcome!