I would like to perform an XTT assay with differentiated SH SY5Y cells in 96 wells. However, although I will plate the exact number of cells in each well, I cannot assume that by the end of the differentiation process the number of cells in each well (across the 96-well plate) will be equal. A possible solution is to differentiate the cells in bigger plates and then move them to 96 well plate. The advantage is that I will be able to control the exact number of cell/well. The possible disadvantage is that the procedure is too harsh and most of the cells will not survive. I will be happy and great full to get advice on this matter. Thank you, Einav.
About 5 years back a similar question was on RG and several good answers have been given. I am giving a copy of that question below: Refer that on RG.
Celia van der Merwe
- 17.34
- University of Cape Town
How should I go about plating SH SY5Y cells in a 96 well plate?
Hi all,I am hoping someone has tips on how I should plate my SH SY5Y cells into a 96 well plate as I have limited experience with these smaller wells. Is 5000 cells an adequate amount to plate? And if I leave it for say 24hrs, would the cells not become overgrown by then?Any current protocols that work would be much appreciated.
Dear Jai Ghosh,
Thank you for the prompt response. However, my question is about differentiated SH SY5Y. In essence, the major problem is that if I plate them directly into the 96-well plate I have no control on the final No of cell after differentiation. If I differentiate them in another plate and trasfer them post-differentiation I do not know how many of them will survive the procedure.
I am positive that people have encouter this technical difficuly and overcome it, but I did not find a solid protocol yet. (hence the call for help :))
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