Hello! I was wondering if anybody has a way of making mouse splenocytes last longer once they are made into a single cell suspension. I have RPMI supplemented with heat-inactivated FBS, L-glutamate, P/S, hepes buffer, and sodium pyruvate. Usually my cells start to die after 2-3 days. Would replacing the RPMI the splenocytes are in every day help them to last longer? Thank you.