We want to extract RNA from virus samples, so we need to make everything RNase free. However, DEPC or RNase cleaning products are out of the question for us at the moment because of their cost. Is there any other way to make our solutions and equipment such as pH meter RNase free?
Hi Aliye,
Maybe a strong source of UV light would the job.
https://offers.the-scientist.com/hubfs/Phoseon%20RNase%20White%20Paper/Phoseon%20White%20Paper.pdf
Perhaps you can find a cheaper source option.
Cheers
You can clean the pH meter probe or plasticware by wiping or soaking them in 0.1 M NaOH. But it is recommended that you soak them in DEPC water to remove NaOH.
By the way, you don't have to buy DEPC water, buy DEPC and make your own DEPC water. You can make 5 liter DEPC water by using 5 mL DEPC.
RNAses are a problem but they can be avoided if you work very carefully. RNAses are not just around, but we are the source. Use gloves, disposables and only chemicals that have been used with special care before. Clean water is free from RNAses as well. Clean equipment with NaOH as Sibel recommended and rinse it with water. Ideally you have pipettes that do not come in contact with RNAses. Anything made of metal or glass, heat up to 250 °C for around 2 hours
Cleaning with 1M NaOH should also do the job. Of course dont forget wearing gloves and using fresh/sterilized pipette tips.
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