My ques is is mercuric chloride make in hcl solution or in distill water¿ when we remove percentage and 2 ques is mercuric chloride unit is mg or ml or g.
I wonder why you need to use a highly toxic staining method based on mercuric chloride. It seems that other people use common protein stains such as Coomassie blue or amido black. See for example https://www.scielo.br/j/mioc/a/zcqMRXddx4kGjZBMXQ9tGcm/?lang=en&format=pdf
3/1/22
Dear Sooda,
I agree with Pierre Beguin. Better to avoid using HgCl2 due to its high toxicity and disposal problems. I'm surprised that a reference published as late as 2018 is recommending this method. The reference Dr. Beguin provided should work well. Another option is to use agar in which casein is incorporated instead of gelation. The agar w/ casein will be opaque. If the microbe growing on casein agar makes protease, you'll see clear zones around the colonies on an opaque background, and without adding any chemicals. Use of casein agar for protease detection is described in any good microbiology lab manual. It's an old procedure, but it works.
I hope this information helps you.
Bill Colonna Iowa State University, Ames, Iowa, USA [email protected]
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