I am making kosaric media using artificial seawater in various salinity for microalgae cultivation. At first, I tried to filter the seawater and then add chemicals. After autoclaving, there was some deposits at the bottom of the media. I also tried to autoclave the artificial seawater and media separately, and then let both solutions cooled down and added together in laminar flow. However, once I added the kosaric media into seawater, precipitation was observed and large amount of white crystalline substance at the bottom. Is there any other way to make it?
hello dear,
i think you can first filter your seawater, then add your desired chemicals might be these chemicals react with each other i am not sure but i heard from my friend, he face such this type of problem.
Hello Ahmad Rafiq , thank you for your recommendation. I tried it and it still reacted after I added in the chemicals separately, so I filtered it again to get a clear media. However, the media started to react and form precipitation after a few days of subculturing microalgae. I read some past literature reported that the sodium bicarbonate and sodium hydroxide (which I use to adjust pH of media) are the main culprits in reacting with calcium and magnesium in artificial sea salt. May I know did your friend solve this problem? If yes, how your friend solved it?
Dr Björn Podola
, thanks for your thoughts and suggestion. The algae cultures were just inserted with cotton plug and shaken by the incubator at temperature 24 C.- Badges
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