I'm planning to measure color change (before and after chemical reaction) of colorimetric sensor arrays with circle shape, and to generate color difference map image.
I read in many journals that this job can be done by using ImageJ program. Unfortunately, I can't find the detailed procedure to do this. Can anybody help me to solve this problem? Thank you.
Dear Mr. Luay Shihab,
Thank you for your great response. My basic knowledge about pseudo color image and RGB color image are being upgraded by reading your comment. However, I attached an image here to point out my question.
In the attached image, I know that the last image (at the bottom) is pseudo color image which is created by LUT. In many articles this image is created by Adobe Photoshop while in other articles it is created by ImageJ. I have know idea how they can create this image by using those programs?
As the last image is obtained by subtracting the initial RGB values of grey color image of each sensor with that of after reaction image, it yield numbers (RGB values) which can be converted into certain colors based on LUT. Is there any macros (for ImageJ) to do this conversion (numbers into colors)?
Hi, could you please share the answer? I have the same question
To all, After spending exhaustive times, I found that the answer is subtracting the RGB values of before with after test. Before that, don't forget to do a blank correction for both values. The obtained subtraction value is then simply entered into the Paint program (or even MS Powerpoint) to generate a new color. Taadaa, you have a color different map picture by now.
Stay well and healthy,
Amrun PS: I attached a png file that I made to describe the procedure.
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Biomolecules
- Peptides