I am currently working on LCLs (lymphoblastoid cell lines), which we developed from the B-cells of healthy individuals through EBV transformation. but i want to study the focal adhesion pattern of different LCLs and for that i have to grow them as adherent cell line without changing its normal morphology. so is there any method or protocol so that i can develop adherent call lines from suspension LCLs.
Hello Daya, You may try 5 mM/ml of Phorbol-12-Myristate-13-Acetate (PMA). it works good for monocytes
Lymphoblastoid cell lines has the property to remain as suspension culture. Normally it donot adhere to the surface. However, as Harsh suggested monocytes normally adhere to the surface of culture flask in presence/absence of mitogen.
Do you have any reference to justify that? I am very interested too. THank you
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