Hi all,
I want to make a drug resistant cell line. What will be the easiest but effective way to make a cell line drug resistant. (IC 50 of my compound is 0.2uM)
Thank you in advance for your answers.
Thanks,
Utsav
If your drug has specific targets in the cells then you can maintain cells with low concentration for large number of passages (45-50 at least).
Hi. One way to make a pure strain resistant to a drug, is to make continuous sowings on agar and put a disc impregnated with the substance; until growth appears very close to the disk. At some point a resistant crop must appear.
Hi Utsav
It depends on how long you cell line takes to grow, if it grows quickly then you can easily create a drug resistant line in a few weeks, if they take long you may need a few months.
First determine your baseline MIC value of your cell line. Then you can start by growing them in medium containing 0.1 uM of your compound, in the next passage take the survivors and grow them at 0.2uM, then 0.3uM, then 0.4, till you reach the concentration you would like. Check the resistant MIC now, there should be a 2- 4 fold difference. Then you have a resistant line.
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