Here is what I have found according to your question:
"Chitosan was dried in the oven until a constant weight was observed. Then 5 g of chitosan was dissolved in 500 mL of acetic acid (0.1 M) followed by mild stirring and heating at about 55 ◦ C overnight to form 10 g L−1 chitosan solution (pH 3.99). The solution was then filtered to remove dust and other traces of impurities and was used as a stock solution. The stock solution was then diluted with the acid to a range of concentrations between 2 and 10 g L−1 . Air bubbles were eliminated by keeping the solutions at room temperature for 2 h."
DOI: 10.3906/kim-0901-38
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First, make a solution of glacial acetic acid in water to achieve a pH value under 6. Add chitosan to that solution and stir at a temperature of 60-70 degrees. But, if you have to achieve these amounts, then take 0.3 g of glacial acetic acid and dissolve it in 99.7 g of distilled water. Then add 0.1001 g of chitosan to this solution.
This is what we performed to prepare a solution of Chitosan. Briefly, we took 2 mL of glacial acetic acid in 1000 mL of distilled water to form mixture (A). Then took 2 g of chitosan in 100 mL of mixture (A).
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