How to know if the assembled bacterial genome is circular or linear?

More Ahmer Bin Hafeez's questions See All

How to get the heading of real-time credit card dataset ?

I am working with the Credit card dataset. I am aware of the fact that the Banks and financial institutions do not share the dataset with third-party companies or researchers. I just need to know...

18 February 2021 9,186 3 View

Is there any database or website from where I can get a list of FDA approved drugs for treatment of certain urinary tract infection?

I want to know all prescription drugs which are FDA approved compounds for infection like Urinary Tract Infections along with their causative agents. Can you guide me about any resource, database...

17 December 2020 4,852 3 View

Where can I find papers related to microstructure of inconel alloy 800?

I need to define the microstructure of inconel 800, but I cannot find any literature related to it. Literature is about incoloy 800 mostly which is a bit different. Can anyone tell me if there...

07 December 2020 3,137 3 View

Any Tutorial on Federate learning Algorithm, used in fraud detection ?

Because of GDPR and confidentiality issues, no bank will expose the user's data to third-party researchers, and hence we cannot train our model for real-time fraud detection with real data (What I...

01 November 2020 6,063 3 View

How can I specify the parameters for nano creep?

Which parameters should I employ for nano creep testing of Titanium and Inconel alloys? Should I load it with a constant load rate or strain rate? What should be the maximum load? What should be...

29 October 2020 5,727 4 View

Is there any other microbes (except microalgae) that could efficiently remove nitrogen and phosphorus content from wastewater ?

I was studying the biological treatment (anaerobic) of wastewater biomass. I found that anaerobic bacteria take a comparatively longer time to biodegrade nitrogenous compounds. Is there any other...

28 October 2020 921 3 View

Would you suggest me a formula for calculating energy yield from biohydrogen and biomethane production?

I was looking for a formula to calculated the obtained energy yield via biohydrogen and biomethane production using anaerobic digestion process. The unit is in SI unit (x) mLH2/g VS and (y) mL...

20 October 2020 8,668 5 View

I want to design a equivalent circuit for series feed network...can any one provide me something useful?

I couldn't find it in any books and articles. I want to series feed two-slot antenna arrays but it is not giving me enough bandwidth. I want to design an equivalent circuit for the series feed...

19 October 2020 4,664 3 View

How to simulate force transmissibility in ANSYS?

I've been trying to simulate the force transmissibility experiment based on the setup shown in the attached diagram where the transmissibility is defined as the ratio of transmitted force...

14 October 2020 9,141 2 View

How to find the elastic work using nanoindentation load depth curves?

I am using work of indentation approach to find out the values of hardness of Ti and Inconel alloys using nanoindentation load-depth curves. I can find the formula for total work. Whereas for...

13 October 2020 2,778 5 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Universal bacterial primers targeting a mRNA?

Hi, I'm looking for universal primers targeting a bacterial mRNA to quatify bacterial load by qPCR in a complexe mixture (eukaryote and prokaryote RNA). Ribosomal RNA being depleted, I absolutly...

22 February 2021 9,969 3 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

How can a reference point be fixed to an attachment point in Abaqus?

I am currently trying to copy an assembly feature, that is present in an old version of the model I am using, onto a new model. The feature is a reference point that is fixed to an attachment...

16 February 2021 8,872 2 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

Can I get the design of circular cylinder to fix thermocouple on the surface and cartridge heater in core ?

Respected Scientific Community. I'm interested to carry out experimental studies on a circular cylinder. But even after doing literature review I unable to get the exact design of the cylinder...

10 February 2021 6,221 1 View

Can I use asymmetric PCR amplification on a pool of circular plasmids to increase each plasmid's signal?

Hello, Here is my experiment: I transfect in a plasmid that has been cut once (a linear piece of dsDNA) into human cultured cells. I wait 48hrs to allow the cells to "repair" the plasmid making...

10 February 2021 6,090 3 View

Plasmid amplification in S2 cell?

When co-transfect S2 cell with selection vector (pCoHygro or pCoBlast ) to make a stable cell line, does the gene integrate into the genome or just like E.coli? How do the plasmids amplify within...

09 February 2021 5,423 1 View