Looking for an approach to isotopically 13C/15N label phosphatidylserine (POPS) for a biophysical study of lipid bilayers. Looking for a plausible synthesis method or a collaboration.
Dear All, Can somebody share a diversity and inclusion questionnaire for the business context or military context. Thank you Anuradha
22 July 2024 1,320 4 View
Please share the GC-MS sample preparation of algal biomass. My research is focused and interested in lipid content should I go for whole biomass or extract lipid and then perform the FAME analysis...
14 November 2023 3,946 2 View
Hi, I am working on GMTSAR. I have a query about SBAS outputs. SBAS gives two types of output displacement maps in GRD format and velocity.grd. The SBAS command calls the number of scenes and the...
01 October 2023 1,441 0 View
I generated loops for the missing residues of my pdb structure, referring to the tutorial (https://salilab.org/modeller/wiki/Missing_residues). Still, when I ran the third Python script, I saw...
18 July 2023 5,058 0 View
At the subsidiary level, profit-shifting mechanisms are present, which can lead to the manipulation of subsidiary-level data. Is there a way to control for the same while using subsidiary...
05 May 2023 7,202 1 View
Dear All I am working on a paper on subsidiary performance and the host country's institutional environment. In some papers on GMM PANEL VAR methodology, there is no mention of use of additional...
02 May 2023 2,528 0 View
I made HSA nanoparticles by desolvation method. I took 180mg HSA and dissovled it in 4mL of milliQ water (45mg/ml) and then in the protien solution added 2.5mL of ethanol dropwise, then these...
23 March 2023 5,835 2 View
Whether posting a preprint will affect the publication of the same paper in a journal ?
22 March 2023 4,229 2 View
Dear all, I'm trying to simulate the crumpling of a hollow cylindrical crash box by a dynamic explicit study using abaqus cae. The material of the box is defined as mild steel and a front plate of...
22 February 2023 3,647 4 View
Hi, I have a water simulation box with 116 water molecules. I used TIP4P water model. Now I need to calculate RDF of water-water, RDF of Oxygen-Oxygen and RDF of Hydrogen-Oxygen. I tried to...
05 November 2022 6,386 1 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
I have a Polyjet that has passed its labeled expiration date for 1 year and I'm going to re-start transfection experiments. It is really needed to change it? Transfection efficiency may be lower?
23 July 2024 8,059 1 View
The above are manually labeled extrinsic matrices based on the first image It can be seen that the projection error at the edge is large, while the error at the center is small. What could be the...
23 July 2024 7,479 3 View
We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...
17 July 2024 7,767 3 View
Hello everyone. During my analyses on LC MSMS, the area ratio (Standard Area/Isotopically labelled Standard Area) increases with time for the quality control point. I'm having trouble explaining...
08 July 2024 3,150 2 View
The image shows 2 western blots. The first one is of protein A, and the second is for the flag tag that the protein is bound to. The lanes are as follows Ladder, Beads, SAB(unbound), and elution....
01 July 2024 3,183 0 View
Hello! I am planning to collect plant leaf samples for stable isotope analysis. I will be collecting them from the field and storing fresh tissue on ice. I have heard that bringing along a food...
01 July 2024 9,125 1 View
Hi, I´d like to trace the C and N flow in a recirculating aquaculture system by labelling the fish feed with concentrated stable isotopes. From a supplier, I found a product ouf of algae lysate...
19 June 2024 746 0 View
My goal is to see two kinds of particles mixed well in an aggregated form. My experiment was as follows: 1. Prepared Microparticles 1 + EDC/NHS + RhoB, and Microparticles 2 + EDC/NHS + FITC. 2....
19 June 2024 2,680 1 View