How to isolate the quinol pool from the membranes?

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Proteins in novex-Thermo native page marker?

I am running a native gel for SOD assay. I have used the thermo/novex native page marker (Catalog number: LC0725) in the same gel. Strangely I am detecting 7 sod positive band between 66 kDa and...

07 August 2017 3,405 0 View

In vivo cross linking of mycobacterial membrane proteins?

I am trying to purify a membrane complex from mycobacteria. It seems that the interaction is very weak within the complex so I am not able to purify the whole complex. I am thinking to fix the...

08 September 2016 9,928 2 View

Why avidin is required for strep tag purification?

I am working with purification of protein by strep tag. In my protocol there is avidin in the binding buffer. Right now I do not have it. Can I proceed with the purification without adding avidin....

07 August 2016 1,206 3 View

How is GFP plus different compared to GFP?

I am working on tagging a protein with GFP plus. I need to know how it is different from tagging with GFP.

06 July 2016 3,153 2 View

The concentration range of histidine for elution of proteins bound to Ni-NTA.

I am using Ni-NTA method for protein purification. Due to buffer constraints I want to elute the protein with Histidine. Can anyone let me know what conc. of His I should include in my wash and...

06 July 2016 9,718 6 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,822 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,105 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 639 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,493 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,246 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,726 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 500 2 View