Dear Scientist,
I badly need someone's suggestion who are working with RNA from etiolated arabidopsis. In my case I am working with 4day old etiolated Arabidopsis seedling to isolate RNA by using TriZol reagent.( 15 min
1. 100mg/1mL trizol, 15 min incubation
2. 200ul chloroform/1ml trizol
3. 15 min centrifuse@12000rpm @-4CAdd 500ul isopropanol> incubate 15 min @room temp.
4. Add 500ul isopropanol incubate 15 min @room temp.
5. incubate 15 min @room temp.
6. 10 min centrifuse @10000rpm
7.Wash pellet twice with 70% ethanol.
But this case I cant see any pellet... :(
Please I need your valuable suggestion to trouble shoot it out.