Dear Scientist,

I badly need someone's suggestion who are working with RNA from etiolated arabidopsis. In my case I am working with 4day old etiolated Arabidopsis seedling to isolate RNA by using TriZol reagent.( 15 min

1. 100mg/1mL trizol, 15 min incubation

2. 200ul chloroform/1ml trizol

3. 15 min centrifuse@12000rpm @-4CAdd 500ul isopropanol> incubate 15 min @room temp. 

4. Add 500ul isopropanol incubate 15 min @room temp. 

5. incubate 15 min @room temp. 

6. 10 min centrifuse @10000rpm

7.Wash pellet twice with 70% ethanol.

But this case I cant see any pellet... :(

Please I need your valuable suggestion to trouble shoot it out.

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