Hello,

It was easy to get the full length of SbBDAH1 (1520 bp) & SbBADH2 (1517 bp) from cDNA using dream taq from thermo scientific, although there were non specific targets and primer dimer, but I could isolate my target genes from the gel. Unexpectedly I couldn't get my target even didn't change any thing of chemicals, kits, primers, cDNA and PCR machine. I had tested cDNA with a reference gene (137 bp) and it works ok but still can't get the full length of the gene.

Similar questions and discussions