28 April 2016 3 2K Report

does anyone have experience to isolate mononuclear cells from human cord blood by ficoll?

More Forough Fatemi's questions See All
If Purospher STAR RP-18 LiChroCART (HPLC column) is suitable for amino acids?

Hi Dear Researchers, Could anyone please provide me with some information about the HPLC column, specifically the “Purospher STAR RP-18 LiChroCART Cartridge”? I would like to know if it is...

25 June 2024 9,523 1 View

Is micro-CT scan analysis able to detect the oxide layer on a titanium sheet or cylinder? what about determining its thickness?

I have a cylindrical sample made from Ti6Al4V powder by SPS technique. I want to coat the lateral surface of this cylinder and measure the coating's thickness, but before the coating process i did...

17 September 2023 9,195 0 View

Do I have any chance to purify some DMSO that mixed with water ?

(I'm not sure about how much water was added)

15 July 2023 9,457 6 View

What can i use instead of POCl3 in organic synthesis which somehow could change alcohol to ether?

description is in the file attached.

10 July 2023 2,238 5 View

How can we find the optimum concentration of a specific powder for EPD?

Hello to all, These days I am focusing on the issue of electrophoretic deposition of different ceramic and metal particles for various coating applications. I have read a lot about the important...

07 February 2023 1,738 1 View

What is the reason of negative response in 3D plots of Central Composite Design?

I used Central Composite Design to optimize effective factors for a typical determination. Now, as shown in attached Figure, in 3D response surface plots, response (peak area) is started from...

01 April 2022 8,672 0 View

How to organize data in Excel Dataset or SPSS Dataset for Fuzzy Cognitive Map?

Dear Colleagues, I am trying to understand the Fuzzy Cognitive Mapping for Urban Risk Analysis. However, I have no idea how to organize my collected data in Excel Dataset or SPSS Dataset to...

09 March 2022 8,436 3 View

Can your recommend algorithms for finding frequent itemsets in sequences?

I'm looking for finding frequent itemsets in sequences, which means the order of appearance of items matters in itemsets. Consider the following example : 1,2,3 1,3,2 3,1,2 Assume that the order...

06 December 2020 2,664 3 View

In clustering, How are graphs of KNN built?

I’ve found out several clustering algorithms such as chameleon or even Spectral Clustering. its work to converting data to a weighted. KNN graph based on the distance between points. I'd like to...

09 October 2019 6,802 4 View

Should the working memory capacity of EFL learners be measured by L1 or L2 memory span test?

The capacity of the working memory is commonly measured by asking participants to remember a list of items and then counting how many letters they can repeat. I have noticed that for some L2...

26 August 2019 2,679 2 View

Similar questions and discussions
Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close