How to introduce siRNA in human astrocyte (no primary) cultures?

17 October 2023 1 683 Report

Hi everyone. Does anyone try to transfect human astrocytes in culture (no primary) to introduce siRNA in order to silence gene expression? Which one would be the best option do do it effectively? Thank yoy

Lautaro Natali

If you already have the siRNAs and what you are looking for is only the method to transfect the cells I would suggest using Lipofectamine RNAiMAX. It has always worked perfectly for me with different cell types and I managed to achieve really good silencing of genes.

If you also need the siRNAs I would suggest to go with siPOOLs https://www.sitoolsbiotech.com/sipools.php. I have also had a really good experience with them.

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