Hello everyone!
Part of my PhD was done in collaboration with a researcher from abroad and, reviewing some methylation data via PSQ-PCR, I realized that I don't know exactly how to interpret these data since they are expressed as "methylation % in CpG positions".
Is there any conversion or calculation that I could do to transform them into absolute numbers and be able to plot the graphs?
I watched some tutorials on youtube, but it's still not clear to me.
I appreciate the collaboration!
Kind regards.
I hope this thread can help you find your answer.
https://www.researchgate.net/post/Can_anyone_explain_how_to_calculate_methylation_level_from_bisulfite_sequencing_results
Please look at the following below links which may help you in your analysis:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3515010/pdf/epi-7-1008.pdf
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2710701/pdf/main.pdf
https://www.nature.com/articles/s41598-020-65406-1.pdf
Thanks
Dear Nivea,
If you are using direct PCR sequencing (BSP) method. you can calculate the methylation percentage for each CpG site based on the file attached.
Best of luck,
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