Can I calculate the average from the data which are collected every 30 minutes for ANOVA?

I artifically elevated the air temperature in the heated plot by 3-celsius degree compared to that in the control for 7 days. I want to figure out the effect of the temperature manipulation on...

21 January 2021 1,185 8 View

What is wrong with my western blot transfer system? (smearing is occured)?

I'm now doing western blotting for several proteins. but transfer is not perfectly happened. bands are looks like smeared or smudged. And it seems to the lower MW bands are more affected. what did...

06 December 2020 9,153 5 View

How to calculate bulk density, porosity, and void ratio of soil?

If the particle diameter is 2 mm and the particle density is 2.5 g/cm, what is the bulk density, porosity, and void ratio of this soil? I had known that the formula to calculate them. The...

11 October 2020 3,466 3 View

How can I check the deparaffinization during IHC protocol?

Hello. I am a beginner researcher experimenting with IHC for the first time. I would like to know how to make sure it is completely deparaffinized when it is fixed with paraffin and removed with...

22 March 2020 7,795 3 View

If the cofactor(NAD) concentration in 1mM and substrate is 10mM in enzyme reaction, the substrate become 9mM after reaction?

If the cofactor(NAD) concentration in 1mM and substrate is 10mM in enzyme reaction, the substrate become 9mM after reaction?

02 March 2020 3,982 6 View

How to make simulated body fluid using glycerol and ethanol?

Hi. I'm trying to find the method making simulated body fluid using gylcerol and ethanol, but I cannot find it. (I want to make the solution that has similar surface tension and viscosity of human...

05 November 2019 9,051 1 View

How many cells do I need to get a pure plasma membrane? Do you have a kit?

The purpose of this study is to obtain plasma membranes from adherent cells by means of ultracentrifuge. The problem with the experiment is that the yield is too low when obtaining plasma...

01 October 2019 3,375 3 View

What is the difference between PET film and biaxially oriented PET film?

Hi. I'm trying to make LIS(Liquid Impregnated Surfaces) using PET film, so I was looking for the appropriate film product. Then I found biaxially oriented PET film, and I want to know if there is...

22 July 2019 329 1 View

Can I use multilevel regression with skewed count data?

I am studying neighbourhood effects on antisocial behaviour using multilevel modelling. The dependent variable, antisocial behaviour is a count data which varies between 0 and 10 which is a skewed...

28 November 2018 8,633 7 View

In sandwich ELISA in human serum, how can I reduce matrix effects and background?

Hello, I'm setting sandwich ELISA to measure amount of target protein in human serum. I have recombinant protein and checked 2 kinds of antibodes(different epitope) were suitable to perform...

29 March 2018 8,728 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Is the box plot of climatic indices better illustrate from daily data or annual data?

Hello, I hope you have a good time. I work on a research project about temperature indices. Due to the high number of indices, I only work on tables and maps on an annual time scale. In other...

19 February 2021 9,742 2 View

What is recommended and opened real-time or nearly real time geospatial data used in flood risk assessment and regional planning on a detailed scale?

I asked recommended real-time or nearly real-time data source for any parameters for flood risk on a microscale analysis for urban area cases in developing countries that had agglomeration urban...

18 February 2021 2,617 2 View

What are backpropagation formulas for residual neural networks?

14 February 2021 4,194 3 View

STS Score - Excel sheet calculator?

I'm looking for an excel sheet to calculate the STS Score. From the website http://riskcalc.sts.org/stswebriskcalc/calculate. Does anyone have a worksheet to calculate the STS Score?

25 January 2021 958 4 View

Is there a paper on the development of the SQFM(AA), the Shorter questionnaire for family members affected by addiction?

I can't find anything on the validation of this tool. It measures stress/strain/coping/support for family members of substance users (or those addicted to gambling).

23 January 2021 1,463 4 View

Is concrete block bricks eco-friendly?

Concrete block bricks companies are marketing that their products are eco-friendly, as they are not emitting carbon dioxide and not using soil. At that viewpoint, they are right. But,in their...

04 January 2021 5,641 8 View

Dear all, Is there any model available to predict the "possible diseases" based on the available pollutant concentration in air ?

In detailed human health risk assessment models. Is there any model that can predict the possible disease.

21 December 2020 7,535 5 View

Can you please answer and share this questionnaire survey about Covid-19 ?

Dear colleagues, Please answer it, will take less than 2 minutes, Please find attached the link below :...

10 December 2020 1,611 6 View

Log 10 transformation versus Benforrini correction?

We have conducted a study to compare the effects of 3 training methods on body composition and cardiovascular risk factors. A Log 10 transformation was applied for all non-normal distributions,...

10 December 2020 877 5 View