It is difficult for beginners to choose the most appropriate measurement method from among the many NMR measurement methods. For example, 2D-NOESY was an immediate trial measurement method for protein researchers, but for natural organic compound experts, the one-dimensional DPFGSE-NOE method is more reliable. Rather than aiming for one correct answer from the beginning, I think it is important to try out the characteristics of many measurement methods with my own sample and then think about it. Please study and learn the fun and depth of NMR.

Many people perform the analysis by the following procedure according to the following procedure. First, DQF-COSY is used to obtain the binding information of the proton. Next, HSQC is used to obtain information on the binding between proton and carbon. Make this a table. Then, HMBC obtains the binding information of proton and carbon 2-3 bond apart. At the same time, obtain information on quaternary carbon. Finally, NOE obtains the spatial coupling information of proton-proton. If these steps do not solve the problem, proceed to more advanced measurement methods, but with many difficulties. It can be said that it is impossible for beginners to analyze compounds having a carbohydrate chain with Polypeptide such as Daptomycin. We recommend that you gain experience with smaller Polypeptides.

Daptomycin is a type protein. You can see the file. Hope it help you.

I greatly appreciate all the suggestions. Thank you.