I have synthesized a 14 or 8 mer peptide coding oligonucleotide DNA to insert in AlwI site of phage ssDNA vector. The synthesized oligonucleotide will have Alwl site at both ends. Important think I have to fuse this oligonucleotide into the ssDNA using AlwI in 5' to 3'. The insertion should be 5' to 3' otherwise it wont be a proper or expected outcome in coat protein. Please give me a suggestion how to synthesis the oligonucleotide with restriction site. The AlwI: 5'-GGATCN3N N-3' & 3'-CCTAN3NN -5'. The planned oligonucleotide with AlwI restriction site : 5'-GGATCN3NN-MSQTSGHS-CCTAN3NN-3'. Please tell me if I digest and insert this oligonucleotide into ssDNA of phage will workout or not. Otherwise tell me the correct way of insertion. Thanks for your help.