e.g., chemicals that can disturb the function of flagellum, or any other methods?
some species with a change in the incubation
temperature range lose their motility
I don't know on what type of bacteria You're working, but there is some research about inhibitors of flagella formation/bacterial motility:
Article The Effect of Inhibitors on the Formation of Flagella by Sal...
https://aac.asm.org/content/55/9/4134.short
Article Inactivation of a putative flagellar motor switch protein Fl...
Article Reversible inhibition of flagella formation after specific i...
Article Inhibition of bacterial motility and spreading via release o...
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