you apply the equation c1v1=c2v2 so if you have 10ul of 0.078ng/ml and you want 150ng/ml then you would have to concentrate the sample to a volume c2 of 10x.078/150 or 0.0052 ul so hopefully you have a much larger volume than 10ul

Harsha Somashekar Why do you want to have that high concentration. Generally DNA concentration is measured in µl. That mean you already have 78 ng/µl and 32 ng/µl. These concentrations are enough for any molecular biology works. You want to make concentration 150 ng/ml that means 150,000 ng/µl. That sounds ridiculously high, and practically not possible.

you apply the equation c1v1=c2v2 so if you have 10ul of 0.078ng/ml and you want 150ng/ml then you would have to concentrate the sample to a volume c2 of 10x.078/150 or 0.0052 ul so hopefully you have a much larger volume than 10ul

Thanks for your valuable answers.

Actually, I am going for Sanger sequencing, for which the plasmid concentration should be at least 150ng/ul. So I wanted to increase the concentration from 78ng/ul and 32ng/ul (Two samples) to 150ng/ul.

Can you re-transform and grow up a culture using chloramphenicol and make a new prep with a much higher concentration to begin with? Or dessicate and resuspend in a smaller volume?

Could you please tell at which step specifically I need to add chloramphenicol? And how this addition increase the plasmid concentration?

You would need to check your plasmid is compatible, but the common ones are. Chloramphenicol suppresses bacterial genome replication while allowing plasmid copy number to increase. Grow the culture as normal overnight or to OD600 0.4, then add chloramphenicol (dissolved in ethanol) to a final concentration of 170ug/mL, then incubate further overnight. I used to get away with inoculating first thing in the morning and adding the chloramphenicol last thing in the evening. Then, extract as normal. Use a non-kit method of plasmid prep or a mega prep kit because you should get a really high yield. This is a pretty ancient classic technique with multiple variations - originally from Maniatis I believe. Bit like Sanger Sequencing - when I first did that I used S35 and panes of window glass held together with packing tape :-D Ah, memories!

For Plasmid specifically. the concentration you have is with in the average range. Since I think that your plasmid purity is good, It is not necessary to reach 150ng/uL to have a good sequencing result.

Article Effects of Plasmid DNA Sizes and Several Other Factors on Tr...

I hope this article benefit for you .

you can use Amicon spin column to concentrate your DNA or protein. it is easy and reliable.