Harsha Somashekar

2 Questions 3 Answers 0 Followers

Questions related from Harsha Somashekar

Why we get to see more than 3 bands on the gel in heterozygous individuals, although, we used only 3 primers for PCR?

To track the Tos17 insertion mutation, I used 3 primers (1 forward and 1 reverse away from the insertion and 1 forward on the insertion site). In wild type I got single band; however, in...

11 November 2018 4,404 6 View

How to Increase Plasmid DNA concentration?

I have two DNA sample, one with a concentration of ~0.078 ng/ml and the other one with 0.032 ng/ml. I want to increase the concentration to ~0.150 ng/ml. Any suggestions are appreciated Thanks...

11 November 2018 678 10 View