When an ShRNA Knock down efficiency is not good, how I should improve this as I know ShRNA KD efficiency is not dose dependent.. it is meaningless to increase the amount of ShRNA right?
Hi Sya Retno
Yes, you can use higher concentrations, but you have to consider also the toxicity.
In addition, use different intervals for transfection if you transfect double or triple.
Dear Sya Retno
I revised some articles discussing the issue and wanna point out the following points:
1. Higher RNAi concentration will cause higher transfection efficiency but not higher inhibition rate.
2. Higher RNAi concentration will result in off-target effects.
3. Of note, the mentioned article considered the inhibitory rate by RT-qPCR only, which may not be the ideal way in this case and can give miss-leading information.
Here, if you would like to achieve higher inhibitory rate, pls consider different intervals and multiple transfection (e.g: 24h, 36h and 48 and repeat the transfection with same intervals).
Hope that was helpful.
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