How to perform R^2 and p-value in Pcoa Plot?

Dear All, I have the weighted and unweighted distance matrix output file from QIIME2. I would like to find the Community structure differed significantly between the groups according to the beta...

08 September 2019 3,016 3 View

Clarification on Proteome Discoverer Output file?

We have done LC/MS-MS analysis and executed Proteome Discoverer for identification of proteins. In the output, I found there are many proteins fragments and also found that one protein present...

10 November 2018 4,299 5 View

How to find significantly expressed proteins from proteome discoverer ??

Dear all, i am a new baby in the field of proteomic. Now I have the multiconcencous results obtained from proteome discoverer. I need to find the significantly expressed proteins between three...

05 June 2018 3,250 2 View

How to assign Taxonomic Classification for OTU tables?

I am new to metagenomics. I downloaded a 16s rRNA data from sra and generated OTU table using USEARCH. How to assign Taxonomic classification to OTU table. I tried with the following...

01 February 2018 2,957 2 View

What is the exact workflow of finding alternative splicing through RNA Seq

I am working on identifying tissue specific alternative splicing event in plants e.g rice. I have done till mapping to the reference genome. I am stuck to continue from the mapping results.

07 August 2017 3,587 1 View

What is skipped exon annotations file?

07 August 2017 7,829 1 View

How to convert .maegz format to PDB format ?

I have a docking results of one protein and one ligand in .maegz format. I need to visualise this result in discovery studio visualiser. But DS visualizer doesn't support .maegz format. So how to...

02 March 2017 6,190 6 View

How to get the complete graph after extened the nanoseconds from 10-20?

I have extended the simulation from 10ns to 20ns using .cpt file during molecular dynamics run,when i am trying to analyze the result i am getting the graph only from 10ns to 20ns instead of...

01 February 2017 2,464 3 View

Is there any idea for creating a perl module

I have assigned to create a small Perl module which can be using for bioinformatics and bio statistics. I have searched for many ideas. But almost all ideas are already there in CPAN. Can anyone...

09 October 2016 7,499 4 View

Summer Internship for M.tech Bioinformatics with 2 months

I am currently pursuing my M.tech Bioinformatics. I am seeking for good internship where i can enhance my Bioinformatics skills. Ive come across so many internship. But i dont know what will be...

07 August 2016 8,024 2 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View