Can anyone let me know where I can get the Cycloviolacin O2 (chemically synthesized) ?

Cycloviolacin O2 is a cyclic peptide that has three disulphide bonds and reported for various biological activities. I am looking for the sources where I can get the chemically synthesized...

21 November 2019 423 3 View

Other than 1,8-naphthalimide dyes, is there any other molecules which can absorb two-photons at a time?

Other than 1,8-naphthalimide dyes, is there any other molecules which can absorb two-photons at a time with possible functional groups which allows modifications

21 May 2018 7,499 8 View

What is the precision of Matlab?

By observing in articles belong to Meta-heuristic algorithms, the best and mean solutions are like 10^{-150} or 10^{-200}. Are they right. What I observed, Matlab considers 10^{-324} equal to...

19 April 2018 8,267 4 View

Hello, Does someone know how to evaluate the intensity of DAB in IHC and the intensity of other chemical staining?

I currently study about the Stroma of pancreatic ductal adenocarcinoma. Therefore I had to deal with various Extra cellular matrix stainings using both IHC and chemical staining methods....

14 February 2018 8,755 1 View

Is it possible to observe protein aggregation using UV - Vis spectrophotometer?

02 April 2017 2,665 8 View

What is the best method to purify basic proteins with pi 7.8?

13 April 2015 5,256 5 View

How can I increase the binding affinity of GST-tagged protein to GST sepharose column?

01 March 2015 7,678 4 View

Why is my protein getting precipitated, even at low concentrations?

I am purifying a recombinant protein (host Rosetta) with his tag. I am getting reasonably good yield (4-5 mg/liter of culture). However after affinity purification using Ni-NTA column, during...

19 June 2014 9,136 4 View

Can anyone help me figure out why I am losing protein during ion exchange chromatography?

I am purifying a protein from a bacterial system and I was able to successfully purify the protein through an affinity column. My protein has a pI of 5.9 and it is stable at higher pH. After...

01 June 2014 8,858 5 View

How much of Dnase can be added and what buffer can be used for cell lysis during protein extraction?

I am isolating protein from more volume of culture. However I am unaware how much dnase to use and also what buffer to use for its activity, if I am dissolving the pellet in 30ml of lysis buffer...

02 July 2013 7,943 4 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Any suggestions for a website that allows you to create questionnaires and gather unlimited data (Totally free)?

I have found only four of them; www.surveyplanet.com www.online-anket.gen.tr www.surveey.com and of course google forms. Expecting to see your suggestions if there is any. Thank you

28 February 2021 3,237 3 View

Do I need to ask permission to use pictures for my questionnaire?

I would like to use some photo from a government website for my questionnaire. But, different photo is from the different owner. So, should I just get permission from that government body or still...

23 February 2021 1,631 3 View

How can I get the coding gene sequences of Hela and U2OS cell lines? Is mRNA sequence of Hsp90 alpha tissue specific??

I am looking for hsp90 mRNA sequence in Hela and U2OS cell lines, I have tried through ncbi but always the output is hsp90 in homo sapiens not in the specific cell line. Kindly, could any one...

22 February 2021 1,853 3 View

How do you interpret multiple meta-regression in STATA?

Please could someone recommend a resource that explains performing meta-regression with multiple moderators. I have read the STATA META ANALYSIS REFERENCE MANUAL, but this only gives examples of...

17 February 2021 7,376 1 View

Can anyone pls provide me information regarding the libraries where from i can get the absorption data of different materials used in solar cells??

I want to create .abs file ( absorption) for simulating solar cell in SCAPS. Can anyone pls provide me information regarding the libraries (websites) where from i can get the absorption data of...

10 February 2021 6,034 7 View

Glucose 6 phosphate dehydrogenase deficiency & NSAIDs - is this combination harmless ?

G6PD deficiency is quite common in our anesthesia practice in the middle east. Intraop/postop analgesia in them is an issue here. Many websites/books give contrasting advise with former advocating...

10 February 2021 4,817 3 View

Up to what extent website personalization can help in creating urge to buy impulsively?

Up to what extent website personalization can help in creating urge to buy impulsively? The digital transformation is making most of the organizations to go online and start doing business through...

09 February 2021 4,430 6 View