Color at the test line of a lateral flow imuunoassay strip ?

Hi, I am trying to develop a competitive lateral flow assay to detect a protein. I coat the protein on the test line and use gold nanoparticle-antibody as the conjugate that binds to the test...

14 February 2019 2,153 4 View

What is the characteristic UV-Vis Spectrum of Ultra small Gold Nanoparticles ?

Hi, I am trying to synthesize ultra small 2nm gold nanoparticles by NaBH4 reduction (Brust Method). What should be the characteristic UV-Vis spectrum of 2nm size gold nanoparticles. Should we or...

18 September 2018 4,460 9 View

What are the ways to inactivate a Virus without affecting its ability to bind to its antibody ?

We are trying to detect a Virus using a sandwich lateral flow assay. We are targeting the epitopes on the envelop protein of the virus. Is there anyway I can inactivate the disinfect the virus,...

15 March 2018 2,631 3 View

How much more difficult it is to detect a whole virus when compared to its Non Structural (NS) Protein ?

I am trying to develop a gold nanoparticle based lateral flow sensor to detect a whole intact Virus. I have tried with 3 different antibodies of the virus. But, I was not successful. Reading the...

08 February 2018 3,611 8 View

Is there any ideal initiator for synthesis of PMMA Nanoparticles through emulsion radical polymerisation ?

I would like to know whether ammonium per sulfate (APS) is a good radical initiator for synthesizing PMMA nanoparticles by emulsion radical polymerisation. Or, does it have any drawbacks...

04 October 2016 7,812 8 View

What Quantum dots would be ideal for highly sensitive paper based lateral flow assay?

Can anyone who worked with quantum dots suggest what be the ideal quantum dot to use for paper based lateral flow assay. I should be able to conjugate antibody to it and we are looking for a...

17 August 2016 7,636 3 View

Can anyone help me with designing a portable sensor using a nitrocellulose membrane ?

I need to develop a portable low cost sensor using a nitrocellulose membrane (NM). I have done an assay for my analyte on a 96 well plate using monoclonal and polyclonol antibodies and a labeling...

31 May 2016 9,650 1 View

Any advice for absorption spectrum coming in the negative region of the y-axis in a UV-vis spectrophotometer?

In a UV-vis apectrophotometer, what does it mean if the absorption spectrum is coming in the negative region of the y-axis (absorbance) even after taking a baseline with reference?

25 December 2013 3,859 21 View

Can anyone help me with design of paper strip for lateral flow immunoassay ?

I have to develop a paper strip for detection of a small molecule, a nucleoside. I need some suggestions on the arrangement of different pads as I am doing the paper based sensor for the first...

01 January 1970 4,405 7 View

Is Lateral Flow Assay specific to clonality of the anitbody ?

01 January 1970 7,976 12 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

How to run repeated measures in mvabund?

I have a dataset of fish species abundances collected from 6 different habitats over the course of 30 years. Fish were collected multiple times per year and environmental variables were recorded...

21 February 2021 3,838 1 View

Is there a method for evaluating nanoparticle adhesion to epithelial tissue in vitro?

I want to measure how well nanoparticles can transfect cells based on NP adhesion properties. In the literature I find people do it in animal studies, and epithelial adhesion helps in transfection...

21 February 2021 7,791 3 View

Any Homemade Organic Synthesis Practices?

Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...

18 February 2021 2,769 4 View

How to get rough estimation of the concentrations of Oxygen and moisture inside the glove box?

Dear colleagues, I was just wondering if you can suggest a good method to get a rough estimation to the concentration of O2 and H2O inside the glove box as unfortunately, our glove box doesn't...

17 February 2021 1,012 3 View

What is the minimum number of studies in each category required for a multiple subgroup analysis to be viable in a meta-analysis?

Cochrane Handbook mentions subgroup analyses are only warranted if there are more than 10 studies (I have 28), and if they are distributed evenly among categories of subgroup. I am wondering if...

17 February 2021 3,467 4 View

Which CGRP I could use to treat murine cells in vitro?

Does anyone worked with CGRP on mice. I m stuck with which the product good to use. I found Sigma products are good but Sigma has a lot of CGRP products and papers didnt mention the code or cat#...

16 February 2021 8,344 2 View