I work with ATCC BAA-903, an anaerobic sulfate reducing bacteria. We have a small anaerobic chamber in our lab. It works with nitrogen flow. whenever I work in the chamber, I turn the nitrogen tank on till the sensor gets to 0.0 % Oxygen. All other times the nitrogen tank is off and oxygen percent varies between 2-10%. I usually prepare my media and place it in a bottle in the chamber. I add my media to tubes every week inside the chamber using sterilized puppet tips that are in the chamber. Last week I saw something white on the surface of my media and walls of the bottle. I made new media and this happened again today. I sterilize everything with 70% ethanol in my chamber. I have no idea what the source could be. I don't know if it is from the instruments or the air inside the chamber. I use high purity nitrogen tank. Something that worries me a lot is that if these contaminations pathogenic or dangerous?!?
Dear Farzaneh,if your media bottle and other materials are little enough that they can enter a autoklave, l suggest you to use autoclave for sterilization. l think,70% alchole can not be enough for sterilization in certain conditions especially in long term studies. Please inform us about results.
Azadeh Farzaneh : I absolutely agree with Dr. Olcayto Keskinkan. 70 % alcohol cannot be efficient sterilizer for many items as bottle and instruments. I recommend sterilization by heat in autoclave. From my experience also sterilization of your anaerobic cabinet with UV light would help, if the contamination comes from the gaseous atmosphere.
Best regards
Vit
Thank you Dr. Keskinkan and Dr. Mateju, I always sterilize my instrument before putting in the chamber. once they are in the chamber I use 70% ethanol overtime I work in there. Is there a possibility that the contamination be pathogenic or dangerous?
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