Can any one help me in figuring out the methods or techniques in determining the host switch events between virus and host.
Can I get brief explanation about this this theorem, which is applied in venturi meter ?
25 October 2023 2,438 9 View
Hi, I have been working on lipid extraction from marine microalgae using hexane/ethanol as the solvent. I am facing precipitation issue during the final part of the lipid extraction, which is the...
07 May 2023 5,964 0 View
Has per our reference we made 58 mM stock of reagent metol and for working we have to take 1.93 mM from stock, what is the method to take working or any dilution factor or formula available ?
23 November 2022 3,676 0 View
A test was carried out in a final 3 ml reagent mixture containing 1.93 mM Metol, 68.6 lM copper and 1 mM acetic acid/sodium acetate buffer of pH 5.4.
12 November 2022 8,249 9 View
Hello everyone, I'm trying to perform Proteinase K assay for downstream analysis of the mitochondrial localization of a protein of interest. I isolated mitochondria from HeLa cells (using...
18 October 2022 2,106 4 View
I am working on developing a POC (lateral flow immunoassay) which uses blood as sample. The problem I face is that when the Test is initiated, the Nitrocellulose pad is having the blood particles...
12 April 2022 8,701 1 View
In blast furnace ,pool type runner is used to reduce the frequent patch up of runner while furnace is in running condition.Whether any instrument is available to measure the runner wall thickness...
27 February 2022 6,154 2 View
I am looking for a simple vibracoring equipment for sampling wetlands, any leads from my friends and colleagues in India will be appreciated, thank you. Would be happy to import too.
21 December 2021 9,168 1 View
Dear all, I am a student of Master of Business Administration and I have to submit a thesis for the completion my degree. I am trying to understand the current issues faced in the field of...
31 March 2020 752 4 View
Can you suggest an accurate method for measuring the bandgap of solution synthesized quantum dots.
13 March 2019 8,833 5 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
What is human-computer interaction (HCI)?
22 July 2024 10,056 2 View
What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?
15 July 2024 1,598 3 View
Hello! everyone, I am trying to study in silico Protein-Protein and Protein-RNA interactions. Now, is there any tool with which I can identify the interacting amino acid residues or the...
14 July 2024 950 2 View
I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...
14 July 2024 8,482 3 View
I'd like to know if anyone has got experience in intracerebroventricular (ICV) AAV inj. I am looking for a concomitant spread of the AAV including but not limited to the motor cortex and the...
12 July 2024 781 0 View
I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...
11 July 2024 5,669 5 View
Hey everyone, I've been working with GRIN lenses (Inscopix) for almost 1 year and still had no luck seeing strong fluorescence on them. When I look at them, I can see good blood flow and,...
08 July 2024 5,178 2 View
I get two different bands from BCMV after PCR
07 July 2024 2,112 1 View
We have a couple of primers but when we make a blast for those primers we see two results: When I use Blast from NCBI, I found many lineages which anniling with many lineages. But when I want to...
01 July 2024 7,644 3 View