What is the gyan (enlightened knowledge, understanding) on calculating the quantity of ligands required to functionalize any particular nanoparticle?

There are many procedures out there: TGA, ICP-MS, NMR these are all post process. But what is a good method to estimate the extent of ligand required for functionalization? If we know the size of...

22 March 2018 9,772 3 View

What could be the possible reasons for getting 20 unit less value of mass of pure compound which is well charecterised by NMR?

My compound is well charecterised by NMR but in case of ESI-mass spectrum it is coming 20 unit less then the expected mass. Molecule is not having any associatio with water. I could not find any...

12 September 2017 6,260 2 View

How the surface oxide layer involved in the corrosion resistance?

How the surface oxide layer involved in the corrosion resistance?

19 June 2016 5,808 10 View

Is curcumin a good candidate for wound healing besides its cytotoxic effect on Keratinocytes and fibroblasts?

29 December 2015 7,263 4 View

Phylogenetic analysis of the gain/loss of genes along different species lineages?

To identify the gain/loss of genes in a set of species? For example, I am interested in identifying the list of tRNA genes which are gained or lost among different strains of bacterial species or...

17 June 2013 1,261 3 View

How do I find shine dalgarno sequences for prokaryotes?

Is there any tool to identify it or Is there any database with relevant information on it?

18 March 2013 8,708 33 View

I want to learn correspondence analysis and multivariate analysis?

If the above concept is explained in biological example, it will be more helpful for me.

03 June 2012 330 2 View

Is there any database to get the highly expressed and poorly expressed genes?

For any species?

03 June 2012 6,341 3 View

Impact test analysis...

hi... i am trying impact test analysis using composite materials. whether the layup i have assigned material data, also assigned mass point load for rigid discrete model part. am getting error...

01 January 1970 1,826 6 View

Compiler error... how to solve?

hi everyone... as already said before i have tried with so many version to link with abaqus, Visual studio -2016 and parallel XE 19 end with error, visual studio-2015, fortran-16.0 end with...

01 January 1970 2,754 6 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

How to build fluid structure interaction simulation for elastic ring squeeze film damper in COMSOL?

How to build fluid structure interaction simulation for elastic ring squeeze film damper in COMSOL?

22 February 2021 5,408 1 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View