Hi,
I tried multiple aminoacid alignement (with homologous crystalized enzymes) using Clustal omega to identify the glutamate residues. The alignement revealed the presence of several conserved motifs. But I am not able to know which Glu in my enzyme is involved in the catalytic activity.
Any help would be greatly appreciated.
Thank you in advance.
Jihene Maati Assalam o alaikum wa rahmatullabhi wa barkatuhu
Hope you doing well
As you said about the catalytic region in which Glu is present or not? So, you should go through the experimental article on that enzyme targeted and read it thoroughly about the catalytic site and search for your required residue. On the other hand, many servers are available to figure out the catalytic site so you also can go through it.
https://prosite.expasy.org/
http://sts.bioe.uic.edu/castp/index.html?2was
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