Jihene Maati

2 Questions 1 Answers 0 Followers

Questions related from Jihene Maati

How to identify in silico the catalytic glutamate residues ( acid/base residue and nucleophile) of my enzyme ?

Hi, I tried multiple aminoacid alignement (with homologous crystalized enzymes) using Clustal omega to identify the glutamate residues. The alignement revealed the presence of several conserved...

10 November 2022 1,447 1 View

How to renaturate my protein after urea treatment?

Hi, I have made a purification under denaturating condition using urea. Any suggestions for protein refolding following elution? Thank you in advance.

12 January 2022 9,904 5 View