Chromatin extraction protocol from 3D spheroid cells for CHIP application?

Hi.. I'm wondering if anyone can recommend a protocol to extract chromatin from 3D spheroid cells. I have glioblastoma patient derived (GBM) cell line that turns into spheroid and I want to...

11 December 2019 7,942 2 View

How can I assess xylanase activity from a powder form enzyme ?

I'm using a protocol proposed by Bailey et al. 1992 to measure xylanase activity from a liquid form of enzymes in this protocol I use a dose = 0.1 ml of enzyme . I'm wondering if I can use the...

12 October 2016 4,163 4 View

Hemicellulose HPLC protocol?

I want to know the best solvent to characterize hemicellulose by high-performance liquid chromatography HPLC

11 January 2021 4,677 3 View

I want to convert Methane gas to liquid Methanol using photocatalysis. Can you please suggest me a good paper which I can study ?

There are a number of publications regarding this, but none of them are clear and show the mechanism of gaseous Methane conversion to liquid Methanol.

06 January 2021 364 3 View

Can "Natural Language Processing" be used for modelling information flow between platforms?

Dear fellows, I am working on my bachelor thesis right now. My vision was to extract some sort of information flow between platforms. Concretely, I wanted to analyse 4chan /pol/ posts from 2016...

05 January 2021 9,424 3 View

Can i know the conversion of human SAR value to that of a mice?

The phone have 'Head SAR' of 0,527 W/kg, and I give exposure to male Mus Musculus 3G (2100 MHz) and 4G (1800MHz) each group for 1 hour per day for 30 day. Is the result will be the same in human?...

26 December 2020 2,475 3 View

How to calculate the flow rate required for fluidization for a fluidized bed gasifier ?

We need to calculate the flow rate of air required for fluidization of the bed. We have a compressor which gives air at 7 bar and a 1 bar rotameter for measuring the flow rate in LPM. How can I...

16 December 2020 2,092 3 View

Can Apparent Activation Energy determine Conversion Rate in a catalysis reaction?

Recently, I got a concept that is contrary to my understanding from two papers. It can be described as follows, in brief: There are two catalysts for the same reaction, on the catalyst with higher...

09 December 2020 2,406 28 View

How to convert Unit/mL enzyme concentration to FPU/mL or FPU/g concentration?

Is there any conversion formula for this purpose.

06 December 2020 3,283 3 View

Any simplified protocol or methods of estimation of cellulose, hemicellulose and lignin content in a biomass?

Dear all, Kindly suggest me a simplified/easy method of calculation of cellulose, hemicellulose and lignin content of agricultural biomass along with the relevant reference. We have used the NREL...

02 December 2020 4,362 3 View

Is it possible to add hierarchical porous Cu/Zn-nanoparticle@carbon materials into a carbon paper based gas diffusion electrode (GDE) ?

26 November 2020 5,075 1 View

How to inhibit the ion conversion between Mn4 + and MN2 +?

Dear scientists, I have a question to ask you, it is known that Mn4+ is beneficial to the soil, but part of Mn4+ in the soil is easy to be converted to MN2 +, is there any way to inhibit its ion...

21 November 2020 1,200 5 View