How much cell pressure and back pressure should I apply to start for triaxial test for different types of soil?

We have recently purchased a triaxial test instrument (MATEST) for testing different types of soil. After installing software, we are facing a issue of saturation where the samples does not...

23 February 2021 7,194 4 View

How to produce huge amount of protoplasts from Fusarium graminearum?

Hi, all I am using some protocol for protoplast production from Fusarium graminearum (Fg), but I need them in a huge amount. Sometimes the protocol worked well but most of the time failed....

14 February 2021 2,286 4 View

Whom should be given the priority for vaccination for Covid-19?

Mass vaccination for Covid-19 is going to start very soon. Whom do you think should be given priority for vaccination? Senior citizens, frontline workers or students? Why?

24 December 2020 8,267 6 View

How to do Fusarium graminearum protoplast transformation using desired plasmid?

I would like to specify my question regarding the transformation of plasmid DNA into F graminearum protoplast. I am using the following reaction mixture (30%PEG 50ul, 200ul protoplast, and 20ug of...

14 December 2020 4,251 1 View

How could we redope Ni deposited PPy?

We prapare Ni deposited polypyrrol (PPy). But after Ni deposition, the conductive polymer is depoded and in CV analysis, the observed current value is very low. An advice is expected from the...

02 December 2020 9,204 3 View

Could you explain the following situations, please?

I am trying to simulate a flexible antenna on ANSYS HFSS. Here, I have used "PDMS" as a substrate and "pec" as a probe (inner conductor) of a coaxial feeding. But during simulation, it is showing...

24 November 2020 800 5 View

What technique can be used to monitor carbon dioxide in natural waters?

I am looking for a method or a sensor for the monitoring of the dissolved carbon dioxide in wastewater and natural water.

22 November 2020 3,439 4 View

Can I get an explanation for the following reason, please?

My plan is to fabricate a conformal microstrip patch antenna. I am going through the simulation works first. I already simulated the planar antenna. Now I am trying to simulate it with different...

19 November 2020 2,961 2 View

I want to determine intracellular calcium concentration of Hela cells using Fluo-8 AM? Is there any protocol?

I also want to use Spectro fluorometer to measure calcium concentration. One more thing, after treating cells with calcium dye, Should I lyse the cells or not to measure calcium concentration?...

11 November 2020 7,757 3 View

Is it ok to spin serum at 7000 rmp for ELISA?

I wand to estimate antibody in serum by ELISA. In order to obtain clear serum, is it ok to spin serum at 7000 rpm (without refrigeration) for 5 minutes? Thank you in advance.

08 November 2020 2,456 5 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View