Hi everybody.
I have a problem for growing up cell A549 and Vero cell, it's very slow in the EMEM with 10%FBS. There is any suggest for another choice of medium or technique?
Thanks.
Use RPMI 1640 with 5% FBS plus 1% Glutamine and antibiotics. The doubling time of A549 is approx 24 h in this medium as we observe in our assays.
thank you for the answer Vehary Sakanyan. I will try the medium for growth A549
at fist use RPMI 1640 with 20% FBS until cells will be come better then decrease FBS TO 10%
I use DMEM high glucose (Sigma's) with antibiotics and 10% FBS. A549 grow rather fast in it. Good luck!
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