We’re running live and permeabilised cell based assays on IMR-32s and they get washed off very easily. We’ve switched to pipettes for more delicate aspirating but many cells still die. We’re using DMEM as media.
We also have background red fluorescence for unknown reasons. Would be great to hear suggestions!
Skylar King
You can treat your coverslips with poly-lysine to make them adhere better. We use glass coverslips often. You have to be very careful when washing etc. or the cells will disattach. It helps to slowly release the fluid on the side of the plate to not disrupt them.
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