How to get free activation no for graphpad prism for IC50 Calculation?

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Could you recommend some articles on Urban Transportation System optimization and Innovation?

13 August 2024 2,595 3 View

Is there any cases algae not using the nutrient from the wastewater and grow normally?

I am working on microalgae cultivation using waste water. The initial concentration of nutrients were less but the microalgae has achieved biomass growth of 2 g/L. The final concentration of...

08 August 2024 4,812 2 View

1. If I can quantize the atom using this hyperbolic spiral and classical physics, could nature do the same?

If we map as a continuous motion an ionising electron (beginning its journey at n=1) in an H atom, a specific hyperbolic spiral appears (see animation). When we solve this spiral formula, we find...

07 August 2024 5,343 2 View

Articles on" Gender disparities i leatherwork education"?

Articles on" Gender disparities i leatherwork education"

07 August 2024 2,500 0 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

What should I do with parameters that are not relate to my simulation in MyLake model?

I want to Estimate surface heat fluxes using MyLake, but I don't have all the initial values in model parameters section and other sections,is there a way?

04 August 2024 1,537 1 View

Why reactivity isn't increased with more empty spots in valence shell?

If from a geometric perspective the non-halogens, non-noble gases have more empty spots in their valence shell, and the filling/exiting of any of the empty spots in the shell constitutes a...

03 August 2024 4,787 2 View

Why is the molecule's orientation with an electric field affect polarizability?

Why is the molecule's orientation with an electric field affect polarizability? Electrons are diffuse enough to be independent with respect to orientation and effect of electric field on...

03 August 2024 7,843 1 View

Why don't d-orbitals split themselves, why does it take a ligand? why don't protons from ligand repel nucleus split d-orbitals?

why don't d-orbitals split themselves because of themselves without the presence of ligands? Electrons are indistinguishable. Why wouldn't it be more correct that protons from a ligand split the...

03 August 2024 3,589 3 View

Why doesn't chromium 2+ ion use all its d-orbitals to receive lone pairs from six waters in [Cr(H20)6)]3+?

I'm guessing it's because the ligand experiences too much electron repulsion or proton repulsion from the chromium to insert them close to the 3d-orbitals which are close to the metal nucleus. Is...

03 August 2024 1,370 1 View

How can I calculate IC50 in DPPH test by using graph pad prism software?

How can I calculate IC50 in the DPPH test using graph pad prism software? or suggest a manual method and other software to calculate the IC50 value.

06 August 2024 8,848 3 View

What is the correct method to calculate the IC50 value for the MTT assay?

I had done calculating MTT assay using Slope intercept method - Excel Four parameter logistic regression model-web portal Using Graphpad prism. Among them which one is the best and suitable for...

01 June 2024 2,576 3 View

What is the most appropriate statistical test to perform when comparing the percentage of 2 cohorts that have a particular mutated gene?

I am using GraphPad prism for data visualisation and analysis. I am trying to assess if there is a statistically significant difference between the percentage of 2 cohorts (patients and cell...

17 May 2024 4,424 1 View

How to include statistics at 2 levels on graphpad ?

Dear All I have a question regarding the use of graphpad, prism I have an experiment with a control and a treatment (2 conditions) For each N (1 patient), I took 5 pictures to quantify an effect...

12 May 2024 3,929 2 View

Calcul IC50 rélatif par graphpad prism 10 ?

Bonjour, 1. Comment déterminer l' IC50 rélatif du test MTT par le GraphPad Prism 10 ? En effet, j'ai un résultat pour la dose maximal utilisée est de 60% de perte de viabilité et non pas à 100...

09 May 2024 5,759 2 View

How to calculate IC50 for different populations have anticancer activity?

How to calculate IC50 for different populations have anticancer activity

26 April 2024 5,066 3 View

Can you help me to find over than 30 Cannabis plant derived molecules with IC50 ?

Hello, I am looking for a series of over 30 molecules derived from the cannabis plant with a specific biological activity and the IC50 values of all the molecules. Could you help me find this...

22 April 2024 9,460 0 View

Geisser-Greenhouse approximation underestimating significance?

I have performed a fairly standard kinetics experiment, in which I investigated the stability of various oligonucleotides in an enzyme solution, by incubating the oligonucleotides with the enzyme...

17 April 2024 3,655 8 View

Discrepancy in Cytotoxicity Assessment of MDA-MB231 Cell Line: Seeking Clarification on IC50 Determination?

I'm currently conducting an experiment on the MDA-MB231 cell line, aiming to assess cytotoxicity using the XTT assay to determine the IC50. Initially, I seeded the cells at a density of 7000 cells...

14 April 2024 1,882 1 View

What to do about some data sets being normally distributed, some being normally distributed after log transformation and some not norm. dist. at all?

Hi all, I have collected some data from 3 treatment groups (n=5) with relation to 6 markers of fibrosis. When I was looking to analyse (with GraphPad Prism) these 6 data sets, I noticed that some...

11 April 2024 7,526 3 View

José Luis Duque-Rivera

You can download the Compusyn software for the dose-effect analysis of single or combined drugs free of charge on the website listed below. This also allows the determination of IC50.

http://www.combosyn.com/feature.html

Daniel Carneiro Moreira

You can sign up to use a 30-day trial version of GraphPad for free.

https://www.graphpad.com/demos/

Carlos Bermúdez-Jiménez

Hi, you can use this page online to calculate the IC50 for free, I hope it works for you.

https://www.aatbio.com/tools/ic50-calculator