How to get bigger size Mon Layer MoS2 Using Mechanical exfoliation?

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Cell optimization in 1D Nanotubes?

Dear All, I use Quantum Espresso. I want to optimize the structure of the carbon nanotube using the vc-relax. I got confused about how to fix cell_dofree here. For 3D, cell_dofree = 'all' or...

22 July 2024 5,708 0 View

I want to plot calibration curve of pure mirtazapine powder using HPLC. Can someone please suggest a method without HPLC buffer as a mobile phase?

I am searching for HPLC method to estimate Mirtazapine but i want to avoid HPLC-buffers as a mobile phase component. So, I am looking for alternative HPLC-solvent (acetonitrile, methanol etc.)...

14 July 2024 1,493 3 View

Error of Reshape the data in Pythonplot code?

I am trying to plot using the attached file data file, "wan_band.dat". In the past, I also found the same error. But somehow it was resolved automatically. Again, I stuck with it. I am not able to...

09 April 2024 4,738 1 View

What are the diffrent nonpharmacological treatments mentioned in Ayurveda being in practices ?

List of Nonpharmacological treatments modules cost-effectiveness practices PMC publications

06 February 2024 9,814 2 View

HEK293T cells electroporation?

If anybody has successfully done electroporation of RNA into HEK293T cell, kindly share the electroporation parameters. Or, if anyone is using Maxcyte STx machine for electroporation, please share...

28 January 2024 8,008 0 View

Is blood isolated monocyte can be kept in incubator in which cancer cell lines are growing?

Dear researchers kindly let me know is it will be feasible to culture blood cells in animal cell culture lab and put the isolated cells to grow in cell culture medium in the same CO2 incubator in...

06 January 2024 1,189 5 View

How to prepare fecal slurry/water?

In our ongoing study we aim to assess the fecal microbiome and metabolome in patients in Indian population. For achieving the same we are referring to Franzosa Eric et al's., study entitled "Gut...

11 December 2023 8,754 0 View

Why no RNA formed?

I have isolated plasmid and then linearised it with re. Then I put in-vitro transcription of the linearised plasmid but not band of mRNA could be observed only the linearised plasmid was seen....

19 November 2023 3,607 7 View

How shall I proceed with large plasmids?

What size of plasmid can be isolated from qiagen miniprep and midiprep kit, respectively? And at what temperature shall I grow a 36kb plasmid with 12.5ug/ml chloramphenicol resistance?

07 September 2023 2,600 3 View

Why no bands on Restriction Digestion of 36kb plasmid??

I am trying to isolate BAC plasmid of size 36kb. I tried with Qiagen Miniprep kit and Zymo BAC purification kit and ran on 0.5% gel. The bands can be seen on the gel but no bands were after I did...

31 August 2023 2,932 4 View

How to treat monolayer culture cell line with essential oil?

Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...

31 July 2024 5,301 2 View

How to remove DDQ from bodipy reaction only possible colmun chomatography tecnique use here ?

bodipy moiety are high polar nature .so its are bottom of TLC PALTE up to 20;80(ethyl acetate;hexane) mixture so its binding in SiO2 SO YIELD is so decreased. how to remove DDQ from reaction mixture?

02 July 2024 8,846 4 View

How to calculate the proportions in grams or milliliters of the compounds for 60 mL of SAPO-11 zeolite precursor gel?

It is required to make a precursor gel for a SAPO-11 Zeolite, the compounds are the following: * As a source of Al: Aluminum Isopropoxide (98%) Formula: C9 H21 O3 Al PM: 204.24 g/mol Density:...

01 July 2024 617 3 View

How to transfer a full grown cell monolayer to another plate?

Hi everybody, i need to pick your brains: i work with an adhesive fish cell line which forms a konfluent monolayer. I want to test an adsorbant substance, therefore i want to saturate the cell...

30 June 2024 9,300 0 View

How can i calculate the band alignmet of Sc2CO2/BlueP heterostructure from DFT?

I know the work function and band gap of each monolayer and heterostructure. I calculated mulliken electronegativity(X) of Sc2CO2 and Bluep. From that i found Ev = X- 4.5+Eg Ec = Ev-Eg, where Eg...

28 June 2024 9,360 0 View

Measuring the Raman Spectrum of Monolayer Graphene with a 785 nm laser?

I am trying to obtain the Raman spectrum of monolayer graphene deposited on Si/SiO2 and grown through CVD. I obtained the graphene from ACS. However, the microscope only has a 785 nm laser. From...

19 June 2024 2,148 4 View

Why is SHSY5Y getting killed by protective drug?

I have received passage 29 from our vendor (NCCS, Pune). The cells are slightly different from the previous batch, they form neurospheres, and on adding 12% instead of 10% they show 90% monolayer....

10 June 2024 4,790 0 View

In surfactant modification of zeolites, ECEC and CMC are of primary interest. How do you determine which concentrations to employ?

I've read literature in which the rationale behind their monolayer and bilayer surfactant modification for zeolites was solely based on the external cation exchange capacity (100% and 200% ECEC...

13 May 2024 1,031 2 View

How to make a misorientation of 21.78 degree in MoS2-WS2 heterobilayers using vesta or anything other than vesta?

I am trying to find the thermal conductivity of MoS2-WS2 heterobilayer structure by following https://doi.org/10.1007/s10765-020-02627-6 this paper but I am unable to create a misorientation of...

08 May 2024 528 0 View

Metal film diffuses through SiO2 and reaches Si substrate?

I deposited a 50-nm-thick Pt film on a silicon substrate using magnetron sputtering method. TEM results showed the presence of SiO2 layer between Pt and Si-sub. Strangely, between SiO2 and...

06 May 2024 531 3 View