How to find the sequence under specific SEQ ID NO.: XX in a published patent?

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When to restimulate human T cells?

If I stimulate the PBMC with coated CD3CD28 mAb, should I transfer cells to uncoated plate after 2-3days of stimulateion to avoid over stimulation? When can I restimulate the cells and how should...

10 November 2017 9,970 0 View

Which CD3/CD28 antibody are good for in vitro T cell activation?

Hi I want to use pre-coated CD3 and CD28 mAb to activate T cells. I just realize there are different types and clones to choose. Based on your experience, which antibody works best？from which...

02 March 2017 7,740 9 View

I can't find the sequence labeled in a patent

Hi, I read this in a patent: "the 21 amino acid signal peptide of CD8α (translated from 63 nucleotides at positions 26-88 of GenBank Accession No. NM_001768). The CD8α hinge and transmembrane...

31 December 2016 7,027 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Does anyone know why this article is republished using the fake address. Is it about stealing my patent?

This article is mine. It was republished by unknown person using an address (Wilson N Jones Regional Center, Sherman Texas USA. I have a patent.

10 August 2024 7,528 5 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

Hello Everyone ! I'm looking for a good journal to publish my manuscript with low publication cost?

I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.

06 August 2024 6,796 3 View

Is an invitation to join the editorial board of Clinical Cardiology Updates a scam?

I received an e-mail invitation to join the editorial board of Clinical Cardiology Updates. While I have published a few articles related to cardiovascular disease, there are lots of colleagues...

06 August 2024 8,981 8 View

Why is the Protocol for Preventing Postpartum Hemorrhage (https://pubmed.ncbi.nlm.nih.gov/36113283/ ) not being widely adopted?

If the placenta is delivered in squatting position within 3 minutes of the birth of the baby, postpartum hemorrhage is completely eliminated. It works every time. It is quite likely that if a...

05 August 2024 8,895 0 View

How do i get an account to upload my published papers?

need to open an account to upload my published papers

01 August 2024 9,255 1 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View