What is the best method to conduct a meta analysis?

Dear Colleagues: For those of you who have conducted a couple of meta analyses, do you agree with these steps? What is the best method of extracting the data from the chosen studies? Formulate...

26 October 2020 1,539 6 View

What's the best way to contact you?

I'm working with some folks involved in the Herstory Project - I think they would be interested. [email protected] Thanks.

09 December 2018 3,461 3 View

I'm working with some folks involved in the Herstory Project - I think they would be interested. What's the best way to contact you?

[email protected] Thanks.

09 December 2018 4,784 2 View

People who have successfully cultured E14 mESC (feeder free)- what FBS catalog and lot number did you use?

05 June 2016 8,265 3 View

Can Copper(I) and Copper(II) Alkoxides of such type be synthesised?

Application of Copper(I) and Copper(II) are more in Nanostructured applications

09 October 2014 8,709 2 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

How to improve RNA isolation?

Hi, I have been struggling with a weird issue for my RNA samples. I used the Qiagen RNA plus mini kit. For some reason no matter how careful I am with the washes, when I measure the RNA, I get...

22 February 2021 1,286 6 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

How are wild-type and mutant gene and protein isolations and analysis performed?

Considering that we hypothetically designed an experiment, we have an x gene, and we have the mutant version of this gene with a single nucleotide mutation in one of the exons. What kind of an...

22 January 2021 9,589 12 View

How can I prepare 10mM sodium acetate buffer with pH 9?

I have found an article (Extraction, physicochemical characteristics and functional properties of Mung bean protein) for extracting mung bean protein isolate using water as solvent. As my...

21 January 2021 8,995 3 View

Anybody to help me with Code source for DOUBLE CONCAVE FRICTION PENDULUM BEARINGS Please?

Hello, would you please share the codes for Base Isolation of structures using Double Concave Friction Pendulum Bearings? I am doing a related research but as I am just a beginner in MATLAB, I...

18 January 2021 6,683 1 View

How to extract protein after trizol RNA and back extraction of genomic DNA?

I have limited amount of samples, so I am trying to get RNA, DNA and protein from the same samples. I used Trizol reagent for RNA isolation, then use back extraction buffer for genomic DNA...

13 January 2021 1,566 2 View

Culturing Peziza sp?

Hi everyone, Any successful trials for getting some cultures from Peziza Fruitbodies? We have done several trials using usual medium of PDA/ MA, but all failed. Thanks in advance

29 December 2020 6,966 6 View

Cleaning up single cell RNA from 10x dataset?

Hello, I am new to single cell RNA data processing and am currently running my data through the initial cleanup steps via Seurat on R. I have previously done this on epithelial organoid data and...

23 December 2020 4,709 1 View