I am using Methylene blue in my experiments. Is there any method, other than Clausius-Mossotti's equation to find bulk refractive index of a colored solutions, especially dye solutions?
Here is a paper on photoacoustic refractometry of highly absorbing solutions.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3829540/
Adam sent you a valuable article about P.A. refractometry.
However please note follows:
I am missing this sentence in your question : "concentration range of 5 × 10−6 to 10−4 M of MB solutions in water or other solvent...p. e . " (presence of dye dimers)
Please note that MB (Methylene blue) is always impure (solid state or solutions) and you 'll measure the iindex of a mixture of MB and Azur B and other impurities.
MB and Azur B have a strong ADSORPTION on plastic and glass.
Do you need this method for biological purpose?
Please refine or reformulate your question.
JRG
I use a fiber optic spectrometer to measure the different concentrations of methylene blue solutions (absorbance response). I am trying to understand the contribution of refractive losses and absorption losses.
For a fiber optic based spectrometer, absorbance is due to RI change around the fiber core surface and its inherent absorption of analyte (here it is methylene blue). No I do not have any biological applications as such.
Mr. Manoharan,
Please pay attention to this discussion:
https://www.researchgate.net/post/How_to_derive_a_theoretical_calculation_of_refractive_index_of_the_solution_at_different_concentration
- Badges
- Science topic
- Similar topics
- Chemistry
- Biochemistry
- Biochemical Techniques
- Blotting Techniques
- Immunoblotting
- Dyes