I am working with a plant which i can not grow hydroponically. how can i find the salt tolerant proteins in the plant
With the advent of modern proteomics, we can catalogue almost all the proteins in the living individual and by comparing the control plant proteome with that of the artificial stressed plant proteome, we could come up with the proteins that are expressed in the stressed conditions. Usually 200 mM NaCl is the stress condition for a plant (you have to check for your plant and standardize by giving 100mM, 200mM, 300mM and so on up to 1M if your plant is a halophyte). Compare with the proteome without stress, i.e, control. Characterize the differentially expressed proteins using Mass spectrometry.
Sir, i liked your idea very good. Can u define the procedure more clearely.i am working on halophytes and i have doubt in one plant so i need to do some experiment on this plant
Hello Jagruti Mam, its very nice that you liked my idea. First you have to define the limit to tolerate the salt concentration of your plant of interest. Once you have standardized the stress condition and concentration of your plant, then you need to give stress treatment to your plant by keeping a set of control. You can keep different sets for different time periods. After the stipulated time of your experiment, harvest the molecule (DNA / Protein) from all your experimental sets including control and then do the profiling part. You can do both genomics as well as proteomics from these samples and could come up with some very noble data. For genomics based profiling you can opt for microarray and for proteomics based profiling you need to do 2D gel electrophoresis followed by characterization using MALDI. I hope you would have explained what I was asked.
Salt tolerant gene from mangrove and its associates has already been reported and numerous studies has been carried out in this regard. For your reference, please go through the attachment. Hope it will be useful for you.
Well, Laxmi, in my country the mangrove are in delta of Arabian sea brackish water, however, under the influence of continues rains and flooding conditions they are exposed to fresh water conditions, similarly with high tide and drought conditions they are exposed to saline water of sea. i think protein profiling under these natural varied condition or simulation of these artificially may allow to identify salt tolerant proteins. on a limited number of plants, a patch that an be isolated you may add additional salt to get response over a long exposure.
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