For scientific correct, try two methods, DPPH and beta carotene.

Thank you @Lassaad

no i not agree at all: IS NOT DPPH.

DDPH such any other RADICAL activity test is to test the radical stability or radical scavenger activity.

If you want to test ANTIOXIDAN activity dpph is not the right solution. you have to consider electrochemistry methods, wide choice. Some offer ability to see the value inside the limit of human physiology , particularly to see if your antioxidant is reversible or not, inside this limit.

There is a huge difference between a radical and one oxidizer.

Paride - Milan

DPPH radical scavenging activity assay works well for testing the antioxidant potential of flavonoids.

@Aalami thanks for your answer

@Paride thanks for your response

@kaur thanks for your reply

i have found one article "In Vitro Phytotoxicity and Antioxidant Activity of Selected Flavonoids" and this article confirm the antioxidant activity of some flavonoids such as (luteolin, quercetin, catechol, morin, and catechin) by using DPPH.

The method used for analyzing is depended on your compounds since they work as an antioxidant via diverse mechanisms, i.e:

1. radical scavenger can use DPPH, ABTS

2. metal chelator can use CUPRAX

3. reducing agent can use FRAP

4. lipid inhibition can use beta-carotene

You can used well reported in vitro assays to estimate the scavenging activity for 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), superoxide, hydroxyl, hydrogen peroxide and reductive ability for ferric ions and phosphomolybdate assay.

@Harto thanks for your descriptive answer

@Hunaiti thanks for your elaborative answer

You must use two complementary tests: DPPH assay and FRAP assay to confirm the antioxidant activities of flavonoids

you must also compare your result with a reference compound

DPPH method measure both Hydrogen Atom Transfer (HAT) and Single Electron Transfer (SAT) antioxidant ability making it good for what you want to do. However I recommend you use more than one method considering that each method has its own weakness so two or more methods may help. You may consider adding another method like FRAP. .

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thym Mme Lahnine

Romarin mme Maghazli

Catcus Mme Nabil

[email protected]

envoyer moi vote adresse emel

il y au moins 3 méthodes

DPPH

FRAP

ABTS

Pr Mahrouz mostafa

UCA FSSM Chemistry Marrakech

Different methods make it possible to evaluate antioxidant activity. You need at least two tests to find out the level of antioxidant activity of extract or a pure compound. Some most used methods: DPPH, ABTS, FRAP, TRAP, BCBT etc.

Two good researches give a good answer to your question and they are titled:

1. In vitro antioxidant activity and phenolic contents in methanol extracts from medicinal plants.

2. Antioxidant activity and phenol and flavonoid contents of eight medicinal plants from Western Nepal

ABTS. DMPD, DPPH, and other methods are also possible.

In order to determine the antioxidant activity of Natural products including the Flavonoids, you can use total ferric ion through spectroscopic analysis and 2, 2-diphenyl-1-picrylhydrazyl (DPPH). You can also use FRAP assay or the ferric reducing antioxidant power to evaluate the antioxidant activity.

Reference:

Guleria et al., (1998). In vitro antioxidant activity and phenolic contents in methanol extracts from medicinal plants. Retrieved from https://link.springer.com/10.1007/s13562-012-0105-6

@Catherine thanks

@Md. Nazim Uddin Thanks for you reply

@Luay Thanks Sir

@Mostafa thanks

@Elmi thanks Brother

@Nicolas thanks for your reply

@Hardlife thanks

i have finish the experiment and i used the DPPH and FRAP method to measue the antioxidant activities

https://www.sciencedirect.com/science/article/pii/S1319016412000357

@Omayma thanks

DPPH is good for the radical-scavenging activity. But the Ferric reducing-antioxidant Power (FRAP) assay is able to highlight the reducing-Power of antioxidant flavonoids.

@Nicolas thanks