How to extract RNA from brown adipose tissue(BAT)?

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Chao Shi

Okay，I did a pre－experiment, I get more than 700 ng／μL RNA from about 100 mg BAT.And I have to correct that last time I extract RNA from epididymal fat, I used 200 mg tissue rather

than 900mg as I described before.

Manoranjan Sharma

Dear Shi,

Please find some nicely explained procedure for RNA extraction

Article Highly efficient method for isolation of total RNA from adipose tissue

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5912307/