13 June 2022 4 2K Report

We have measured S11 of biological tissues using slim form open ended coaxial probe. I know Keysight software does this automatically, but if we suppose to calculate relative permittivity and conductivity from S11 by using some algorithm, what approach must be followed?

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What are some good protocols for native outer membrane protein extraction from E. coli?

I am trying to extract any and all outer membrane proteins from a lab strain of e. coli (ATCC 11229) and am looking for the best general protocol to extract outer membrane proteins. All...

25 January 2021 5,736 3 View

Can you do Ponceau staining after loading control for Western Blot?

Hello everyone! I forgot to take a picture of my Ponceau stain prior to starting my first round of primary and secondary antibodies. I'm currently doing my loading control now and was wondering...

25 January 2021 757 3 View

Any instances of when an antibody will bind better if left at RT for days?

Can anyone make sense of this: an antibody was left out for 7 days on the bench at RT and binds better to a metalloprotein than if the antibody was taken out the day of the experiment?

15 April 2020 6,330 3 View

How do I calculate solubility of a compound using a turbidity assay?

I'm planning to measure solubility of my compound in PBS buffer by measuring the turbidity of serially diluted solutions in a microplate reader at 600 nm. Can anyone please inform me how to...

23 November 2019 3,333 3 View

Is anyone using Winepiscope 2.0 epidemiological veterinary medicine software for risk analysis?

The website version is already available but it is time consuming to feed manually. The software is in need very urgent. Please let me know ASAP.

23 October 2019 250 2 View

How to sort out a barcode mix up dilemma in a RNAseq experiment?

Hi there, So I submitted 20 RNA samples to a sequencing facility for bulk RNAseq. I got the results back and I actually suspect there might be a mix up of samples. Out of the desperation, I ran...

04 October 2019 2,801 4 View

Networking - Can you all help me network with former psychologists or counselors from corrections/juvenile detention centers, etc?

I am hoping to find connections with former psychologists and counselor who have worked with correctional populations, addictions, etc in hopes of learning from them about their experiences, any...

30 August 2019 10,008 2 View

What are some reliable methods for validating antibodies?

I want to do a pre-absorption technique, but I'm having trouble finding pure proteins of the following: ASIC 2 NFP (2F11) S46 S100P If anyone knows a good site I can purchase pure proteins or if...

16 August 2019 483 3 View

Has anyone any idea about various softwares that can analyze various aspects of large number of texts?

Is there any one who can give names of softwares that can analyze various aspects of texts?

24 February 2019 7,687 15 View

Can anyone suggest a viral vector encoding for firefly luciferase for transfection into human leukemia cell line MV4-11?

I am currently working on developing a bioluminescent mouse model for investigating the anti-leukemic activity of our test compounds. For that purpose I wish to conduct the lentiviral transfection...

23 January 2019 7,572 3 View

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Do we need to write protocol for a second study of a systematic review that has been registered and published?

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How to increase a model accuracy ?

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Is there a classification scheme for rock cavities or caverns with respect to their size and shape?

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Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

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