Mir SAJAD Rabani

You have one major problem here in making calibration standards - the volatility of acetone and n-hexane. Can you use other fluids? Or use a preparation technique that dilutes the extract in a less-volatile (but miscible with acetone or n-hexane) and prepare your calibration standards in a similar manner. You need to have the appropriate part of the spectrum (UV/VIS/IR) free from interfering peaks.

Please look at the following below links which may help you in your analysis:

https://link.springer.com/content/pdf/10.1007/s00248-006-9082-4.pdf

https://www.cell.com/heliyon/pdf/S2405-8440(21)00439-4.pdf

Thanks

In n-hexane. The best use same batch of n-hex for extraction and calubration.

Regards

GB