Hello,

I do not understand very well. After extracting microRNA with appropriate kit what kind of array did you do? Is that is qPCR array or microarray (hybridization)?

Best regards

targeted array from SA biosciences which is now a Qiagen company. so yes its a qPCR array.

That's what I tought but I was not sure. The diffculty in interpreting, is it due to cq (or ct) values are above 30 cycles. And because of this, the replicates are not homogenous?

Apparently you cannot use SNORD's as the 'housekeeping" control for serum micro RNA. so normalization is becoming difficult. Any ideas how to do this? I have used celegans as an spike in.

You can use software like Genorm, Normfinder...This provides sevral housekeeping (two or more) for normalization. Use a CElegans miR is a good way to get an idea of the performance of the method.

Good analysis.

Dear Anagha

Running microRNAs from serum or any body fluids is always challenging and in our opinion arrays aren't the best solution.

At NXT-Dx, we offer miRNA expression profiling using the latest in qPCR technology. We have compared qPCR,with arrays and sequencing techniques, especially in body fluids such as serum. The qPCR approach clearly comes forward as the optimal solution. On top of this we specialize in low-input approaches with this technique, so are very capable in delivering high quality data from serum samples. We can also take care of the RNA isolation from your serum samples before starting the

If you are still planning to run this experiment and would be interested in learning more about our approach, please send me an email on [email protected].

Maarten Braspenning

NXT-Dx

Dear Maarten,

Where are you located?  can you send me a protocol from your company at [email protected] .  i am interested.

Thanks,

Anagha